이전 Marine genomics에 이어서 조지훈 학생의 연구주제였던 ‘State-of-the-art housekeeping proteins for quantitative western blotting: Revisiting the first draft of the human proteome’ 논문이 2016년 4월 국제학술지 ‘Proteomics’저널에 출판 승인 되었습니다.<br /> <br /> <br /> 이 논문 역시 6월 중 온라인 출판 될 예정입니다.<br /> <br /> <br /> 모두 수고하셨습니다!<br /> <br /> <br /> <br /> <br /> 논문명 : State-of-the-art housekeeping proteins for quantitative western blotting: Revisiting the first draft of the human proteome<br /> <br /> <br /> <br /> 저자 : Hyun-Gwan Lee*, Jihoon Jo∗, Hyun-Hee Hong, Kee K. Kim, Joong-Ki Park, Sung-Jin Cho and Chungoo Park<br /> <br /> <br /> <br /> <br /> 영문초록 : Western blotting (WB) analysis is the most popular and widely used methodology for protein<br /> detection and characterization over recent decades. In accordance with the advancement of<br /> the technologies for the acquisition of WB signals, a quantitative value is used to present<br /> the abundance of target proteins in a complex sample, thereby requiring the use of specific<br /> proteins as internal references that represent total proteins. Heretofore, proteins encoded by<br /> housekeeping genes such as GAPDH, -tubulin and -actin have been commonly used as<br /> loading controls without any hesitation because their mRNA expression levels tend to be<br /> high and constant in many different cells and tissues. Experimentally, however, some of the<br /> housekeeping reference proteins are often displayed with inconsistent expression levels in<br /> both homogeneous and heterogeneous tissues, and, in terms of mRNA levels, they have a<br /> weak correlation to the abundance of proteins. To estimate accurate, reliable, and reproducible<br /> protein quantifications, it is crucial to define appropriate reference controls. For this paper, we<br /> explored the recently released large-scale, human proteomic database ProteomicsDB including<br /> 16 857 liquid chromatography tandem-mass-spectrometry data from 27 human tissues, and<br /> suggest 20 ubiquitously- and constitutively-expressed, putative internal-reference controls for<br /> the quantification of differential protein expressions. Intriguingly, the most commonly used,<br /> known housekeeping genes were entirely excluded in our newly defined candidates. Although<br /> the applications of the candidates under many different biological conditions and in other<br /> organisms are yet to be empirically verified, we propose reliable, potential loading controls for<br /> a WB analysis in this paper.